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Proceedings Paper

3D measurements of live cells via digital holographic microscopy and terahertz spectroscopy
Author(s): Jun Yong Park; Dorian Oser; Peter Iapozzuto; Sean Norbury; Supriya Mahajan; Alexander Khmaladze; Anna Sharikova
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Paper Abstract

This is a study of the central nervous system (CNS) cells, including brain micro vascular endothelial cells (BMV) that constitute the blood brain barrier, and C6 glial cells that are the predominant cell in the brain. The cells are exposed to various chemicals by non-invasive, label-free methods. Digital holographic microscopy (DHM) is a technique that records an interference pattern between an object and reference waves, so that the computationally reconstructed holographic image contains both amplitude and phase information, and 3D images are obtained. The measurement of cell cultures by digital holographic microscopy yields information about cell death mechanisms, since these processes are correlated with individual cell volume. Our in-house DHM combines a visible (red) laser source with a conventional microscope base, and LabVIEW-run data processing. Terahertz spectral signatures are associated with structural changes in molecules and provide complementary information about cells. Both CNS cells BMV and C6 cells are treated with the drug “Methamphetamine" (METH), which induces apoptosis in neuronal cells and exhibits decrease in cell volume, a characteristic of cells undergoing apoptosis (induced cell death). METH can cause CNS cell death by cross-talk between mitochondria-, endoplasmic reticulum-, and receptor-mediated apoptotic events, all of which results in drug induced changes in neuroplasticity and significant neuropathology. Doxorubicin (DOX), a popular anticancer drug, is used as a control. We observe that METH treatment resulted in more pronounced cell volume shrinkage in both the BMV and C6 cells, as compared to DOX-induced cell apoptosis.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97182D (9 March 2016); doi: 10.1117/12.2213765
Show Author Affiliations
Jun Yong Park, SUNY Univ. at Albany (United States)
Dorian Oser, SUNY Univ. at Albany (United States)
Peter Iapozzuto, SUNY Univ. at Albany (United States)
Sean Norbury, SUNY Univ. at Albany (United States)
Supriya Mahajan, SUNY Univ. at Buffalo (United States)
Alexander Khmaladze, SUNY Univ. at Albany (United States)
Anna Sharikova, SUNY Univ. at Albany (United States)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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