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Proceedings Paper

Label-free imaging of gold nanoparticles in single live cells by photoacoustic microscopy
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Paper Abstract

Gold nanoparticles (AuNPs) have been extensively explored as a model nanostructure in nanomedicine and have been widely used to provide advanced biomedical research tools in diagnostic imaging and therapy. Due to the necessity of targeting AuNPs to individual cells, evaluation and visualization of AuNPs in the cellular level is critical to fully understand their interaction with cellular environment. Currently imaging technologies, such as fluorescence microscopy and transmission electron microscopy all have advantages and disadvantages. In this paper, we synthesized AuNPs by femtosecond pulsed laser ablation, modified their surface chemistry through sequential bioconjugation, and targeted the functionalized AuNPs with individual cancer cells. Based on their high optical absorption contrast, we developed a novel, label-free imaging method to evaluate and visualize intracellular AuNPs using photoacoustic microscopy (PAM). Preliminary study shows that the PAM imaging technique is capable of imaging cellular uptake of AuNPs in vivo at single-cell resolution, which provide an important tool for the study of AuNPs in nanomedicine.

Paper Details

Date Published: 15 March 2016
PDF: 6 pages
Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 970857 (15 March 2016); doi: 10.1117/12.2213744
Show Author Affiliations
Chao Tian, Univ. of Michigan (United States)
Wei Qian, IMRA America, Inc. (United States)
Xia Shao, Univ. of Michigan (United States)
Zhixing Xie, Univ. of Michigan (United States)
Xu Cheng, Univ. of Michigan (United States)
Shengchun Liu, Heilongjiang Univ. (China)
Univ. of Michigan (United States)
Qian Cheng, Tongji Univ. (China)
Bing Liu, IMRA America, Inc. (United States)
Xueding Wang, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 9708:
Photons Plus Ultrasound: Imaging and Sensing 2016
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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