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Proceedings Paper

Progress in reflectance confocal microscopy for imaging oral tissues in vivo
Author(s): Gary Peterson; Daniella Karassawa Zanoni; Jocelyn Migliacci; Miguel Cordova; Milind Rajadhyaksha; Snehal Patel
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Paper Abstract

We report progress in development and feasibility testing of reflectance confocal microscopy (RCM) for imaging in the oral cavity of humans. We adapted a small rigid relay telescope (120mm long x 14mm diameter) and a small water immersion objective lens (12mm diameter, NA 0.7) to a commercial handheld RCM scanner (Vivascope 3000, Caliber ID, Rochester NY). This scanner is designed for imaging skin but we adapted the front end (the objective lens and the stepper motor that axially translates) for intra-oral use. This adaption required a new approach to address the loss of the automated stepper motor for acquisition of images in depth. A helical spring-like cap (with a coverslip to contact tissue) was designed for approximately 150 um of travel. Additionally other methods for focusing optics were designed and evaluated. The relay telescope optics is being tested in a clinical setting. With the capture of video and “video-mosaicing”, extended areas can be imaged. The feasibility of imaging oral tissues was initially investigated in volunteers. RCM imaging in buccal mucosa in vivo shows nuclear and cellular detail in the epithelium and epithelial junction, and connective tissue and blood flow in the underlying lamina propria. Similar detail, including filiform and fungiform papillae, can be seen on the tongue in vivo. Clinical testing during head and neck surgery is now in progress and patients are being imaged for both normal tissue and cancerous margins in lip and tongue mucosa.

Paper Details

Date Published: 29 February 2016
PDF: 7 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96891X (29 February 2016); doi: 10.1117/12.2213692
Show Author Affiliations
Gary Peterson, Memorial Sloan-Kettering Cancer Ctr. (United States)
Daniella Karassawa Zanoni, Memorial Sloan-Kettering Cancer Ctr. (United States)
Jocelyn Migliacci, Memorial Sloan-Kettering Cancer Ctr. (United States)
Miguel Cordova, Memorial Sloan-Kettering Cancer Ctr. (United States)
Milind Rajadhyaksha, Memorial Sloan-Kettering Cancer Ctr. (United States)
Snehal Patel, Memorial Sloan-Kettering Cancer Ctr. (United States)


Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong; Justus F. Ilgner; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory; Laura Marcu; Haishan Zeng, Editor(s)

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