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Proceedings Paper

Surface-enhanced coherent Raman scattering (Conference Presentation)
Author(s): Eric O. Potma; Alex Fast; Christropher D. Syme

Paper Abstract

We have developed a coherent Raman scattering microscope that combines total internal reflection illumination with surface plasmon resonance. The excitation geometry is based on an objective-type Kretschmann configuration, which allows widefield excitation of surface plasmon polariton modes in a thin gold film on a glass substrate. The surface plasmon fields enhance the excitation efficiency, enabling image acquisition at 10 frames/s. Since the evanescent field extends only over a length scale of ~100 nm, structures close the substrate surface are observed while bulk contributions are suppressed. We discuss the operational principles of this microscope in detail and point out its applications in cell biology.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120I (27 April 2016); doi: 10.1117/12.2213662
Show Author Affiliations
Eric O. Potma, Univ. of California, Irvine (United States)
Alex Fast, Univ. of California, Irvine (United States)
Christropher D. Syme, Univ. of Glasgow (United Kingdom)

Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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