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Proceedings Paper

Enhanced second-harmonic-generation detection of collagen by means of optical wavefront shaping
Author(s): Jonathan V. Thompson; Graham A. Throckmorton; Brett H. Hokr; Vladislav V. Yakovlev
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Paper Abstract

Second-harmonic generation (SHG) has proven to be an effective method to both image and detect structural variations in fibrillar collagen. The ability to detect these differences is especially useful in studying diseases like cancer and fibrosis.1 SHG techniques have historically been limited by their ability to penetrate and image through strongly scattering tissues. Recently, optical wavefront shaping has enabled light to be focused through highly scattering media such as biological tissue.2-4 This technology also enables us to examine the dependence of second harmonic generation on the spatial phase of the pump laser. Here, we demonstrate that wavefront shaping can be used to enhance the generation of second harmonic light from collagen fibrils even when scattering is low or non-existent.

Paper Details

Date Published: 15 March 2016
PDF: 5 pages
Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97170N (15 March 2016); doi: 10.1117/12.2213644
Show Author Affiliations
Jonathan V. Thompson, Texas A&M Univ. (United States)
Graham A. Throckmorton, Baylor Univ. (United States)
Brett H. Hokr, Texas A&M Univ. (United States)
Vladislav V. Yakovlev, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 9717:
Adaptive Optics and Wavefront Control for Biological Systems II
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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