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Proceedings Paper

4D phase-space multiplexing for fluorescent microscopy
Author(s): Hsiou-Yuan Liu; Jingshan Zhong; Laura Waller
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Paper Abstract

Phase-space measurements enable characterization of second-order spatial coherence properties and can be used for digital aberration removal or 3D position reconstruction. Previous methods use a scanning aperture to measure the phase space spectrogram, which is slow and light inefficient, while also attenuating information about higher-order correlations. We demonstrate a significant improvement of speed and light throughput by incorporating multiplexing techniques into our phase-space imaging system. The scheme implements 2D coded aperture patterning in the Fourier (pupil) plane of a microscope using a Spatial Light Modulator (SLM), while capturing multiple intensity images in real space. We compare various multiplexing schemes to scanning apertures and show that our phase-space reconstructions are accurate for experimental data with biological samples containing many 3D fluorophores.

Paper Details

Date Published: 24 March 2016
PDF: 8 pages
Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200A (24 March 2016); doi: 10.1117/12.2213610
Show Author Affiliations
Hsiou-Yuan Liu, Univ. of California, Berkeley (United States)
Jingshan Zhong, Univ. of California, Berkeley (United States)
Laura Waller, Univ. of California, Berkeley (United States)


Published in SPIE Proceedings Vol. 9720:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management
Kevin K. Tsia; Keisuke Goda, Editor(s)

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