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Proceedings Paper

Wavefront sensorless approaches to adaptive optics for in vivo fluorescence imaging of mouse retina
Author(s): Daniel J. Wahl; Stefano Bonora; Oscar S. Mata; Bengt K. Haunerland; Robert J. Zawadzki; Marinko V. Sarunic; Yifan Jian
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Paper Abstract

Adaptive optics (AO) is necessary to correct aberrations when imaging the mouse eye with high numerical aperture. In order to obtain cellular resolution, we have implemented wavefront sensorless adaptive optics for in vivo fluorescence imaging of mouse retina. Our approach includes a lens-based system and MEMS deformable mirror for aberration correction. The AO system was constructed with a reflectance channel for structural images and fluorescence channel for functional images. The structural imaging was used in real-time for navigation on the retina using landmarks such as blood vessels. We have also implemented a tunable liquid lens to select the retinal layer of interest at which to perform the optimization. At the desired location on the mouse retina, the optimization algorithm used the fluorescence image data to drive a modal hill-climbing algorithm using an intensity or sharpness image quality metric. The optimization requires ~30 seconds to complete a search up to the 20th Zernike mode. In this report, we have demonstrated the AO performance for high-resolution images of the capillaries in a fluorescence angiography. We have also made progress on an approach to AO with pupil segmentation as a possible sensorless technique suitable for small animal retinal imaging. Pupil segmentation AO was implemented on the same ophthalmic system and imaging performance was demonstrated on fluorescent beads with induced aberrations.

Paper Details

Date Published: 15 March 2016
PDF: 6 pages
Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97170A (15 March 2016); doi: 10.1117/12.2213572
Show Author Affiliations
Daniel J. Wahl, Simon Fraser Univ. (Canada)
Stefano Bonora, CNR-Institute for Photonics and Nanotechnology (Italy)
Institute of Physics AS CR v.v.i. (Czech Republic)
Oscar S. Mata, Simon Fraser Univ. (Canada)
Bengt K. Haunerland, Simon Fraser Univ. (Canada)
Robert J. Zawadzki, Univ. of California, Davis (United States)
Marinko V. Sarunic, Simon Fraser Univ. (Canada)
Yifan Jian, Simon Fraser Univ. (Canada)


Published in SPIE Proceedings Vol. 9717:
Adaptive Optics and Wavefront Control for Biological Systems II
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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