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Proceedings Paper

Identification of bacteria causing acute otitis media using Raman microspectroscopy
Author(s): Oscar D. Ayala; Catherine A. Wakeman; Eric P. Skaar; Anita Mahadevan-Jansen
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Paper Abstract

Otitis media (OM) is the leading cause of acute physician visits and prescription of antibiotics for children. Current standard techniques to diagnose acute otitis media (AOM) are limited by their ability to probe only changes in symptoms of the bacterial infection that cause AOM. Furthermore, they are not able to detect the presence of or identify bacteria causing AOM, which is important for diagnosis and proper antibiotic treatment. Our goal is to detect the presence of and identify the pathogens involved in causing AOM based on their biochemical profile using Raman spectroscopy (RS). An inVia confocal Raman microscope (Renishaw) at 785 nm was used to detect bacteria causing AOM in vitro. The three main bacteria that cause AOM, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae were cultured in chocolate agar and Mueller-Hinton agar to determine which agar type would minimize Raman signal from the growth agar. Preliminary results identified specific Raman spectral features characteristic of S. pneumoniae. RS has the potential to accurately diagnose AOM, which will help in identifying the antibiotic that will be most beneficial for the patient and ultimately decrease the course of infection.

Paper Details

Date Published: 8 March 2016
PDF: 6 pages
Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 97040U (8 March 2016); doi: 10.1117/12.2213563
Show Author Affiliations
Oscar D. Ayala, Vanderbilt Univ. (United States)
Catherine A. Wakeman, Vanderbilt Univ. (United States)
Eric P. Skaar, Vanderbilt Univ. (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)


Published in SPIE Proceedings Vol. 9704:
Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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