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Proceedings Paper

Photodynamic antimicrobial chemotherapy (PACT) against oral microorganisms with the use of blue LED associated to curcumin
Author(s): Fernando José Pires Sampaio; Gustavo M. Pires-Santos; Susana C. P. S. de Oliveira; Juliana S. C. Monteiro; Vanderlei S. Bagnato; Antônio L. B. Pinheiro
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Paper Abstract

The use of curcumin as antimicrobial agent has been suggested and this effect may be potentialized by appropriate light. This study evaluated the effect of PACT using blue LED (λ450ηm ± 5ηm, 220mW and spot of 0.785 cm2) associated to Curcumin at different concentrations (75, 37.5, 18.75, 9.37 and 4.68 μg /mL). Microorganisms from the oral mucosa and the posterior region of the tongue were collected and inoculated into test tubes containing 8mL of TSB medium. For these assays were performed 16 readings. In the assays were used culture plate of 24 wells. To each well was added 400 μL of the suspension containing the microorganisms. Suspensions without curcumin were placed in eight wells. Elsewhere, curcumin was applied varying concentrations with pre-irradiation time of 5 min. After stirring, 200 μL aliquot was taken from each well and the readings were immediately carried out by a spectrophotometer (SPECTRA MAX). Assessments of turbidity were performed following CLSI standard methods. After 1 hour of incubation in a bacteriological oven, 200 μL aliquot was removed from the remaining wells for a second reading. The results showed a decrease of total microorganisms in the most of test groups. The best result of the PACT was with 75 μg/mL, showing 81% of inhibition. It is concluded that PACT with blue LED associated to Curcumin could be a potential mechanism for controlling microorganism proliferation on the oral cavity.

Paper Details

Date Published: 8 March 2016
PDF: 6 pages
Proc. SPIE 9695, Mechanisms of Photobiomodulation Therapy XI, 96950O (8 March 2016); doi: 10.1117/12.2213515
Show Author Affiliations
Fernando José Pires Sampaio, Univ. Federal da Bahia (Brazil)
Gustavo M. Pires-Santos, Univ. Federal da Bahia (Brazil)
Susana C. P. S. de Oliveira, Univ. Federal da Bahia (Brazil)
Juliana S. C. Monteiro, Univ. Federal da Bahia (Brazil)
Estate Univ. of Feira de Santana (Brazil)
Vanderlei S. Bagnato, National Institute of Optics and Photonics (Brazil)
Antônio L. B. Pinheiro, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Univ. Camilo Castelo Branco (Brazil)

Published in SPIE Proceedings Vol. 9695:
Mechanisms of Photobiomodulation Therapy XI
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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