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Proceedings Paper

OCT as a convenient tool to assess the quality and application of organotypic retinal samples
Author(s): Rachel Gater; Nicholas Khoshnaw; Dan Nguyen; Alicia J. El Haj; Ying Yang
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Paper Abstract

Eye diseases such as macular degeneration and glaucoma have profound consequences on the quality of human life. Without treatment, these diseases can lead to loss of sight. To develop better treatments for retinal diseases, including cell therapies and drug intervention, establishment of an efficient and reproducible 3D native retinal tissue system, enabled over a prolonged culture duration, will be valuable. The retina is a complex tissue, consisting of ten layers with a different density and cellular composition to each. Uniquely, as a light transmitting tissue, retinal refraction of light differs among the layers, forming a good basis to use optical coherence tomography (OCT) in assessing the layered structure of the retina and its change during the culture and treatments. In this study, we develop a new methodology to generate retinal organotypic tissues and compare two substrates: filter paper and collagen hydrogel, to culture the organotypic tissue. Freshly slaughtered pig eyes have been obtained for use in this study. The layered morphology of intact organotypic retinal tissue cultured on two different substrates has been examined by spectral domain OCT. The viability of the tissues has been examined by live/dead fluorescence dye kit to cross validate the OCT images. For the first time, it is demonstrated that the use of a collagen hydrogel supports the viability of retinal organotypic tissue, capable of prolonged culture up to 2 weeks. OCT is a convenient tool for appraising the quality and application of organotypic retinal samples and is important in the development of current organotypic models.

Paper Details

Date Published: 17 March 2016
PDF: 8 pages
Proc. SPIE 9707, Dynamics and Fluctuations in Biomedical Photonics XIII, 97071C (17 March 2016); doi: 10.1117/12.2213509
Show Author Affiliations
Rachel Gater, Keele Univ. (United Kingdom)
Nicholas Khoshnaw, Keele Univ. (United Kingdom)
Dan Nguyen, Keele Univ. (United Kingdom)
NHS Foundation Trust (United Kingdom)
Alicia J. El Haj, Keele Univ. (United Kingdom)
Ying Yang, Keele Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 9707:
Dynamics and Fluctuations in Biomedical Photonics XIII
Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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