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Proceedings Paper

Prospects and challenges of quantitative phase imaging in tumor cell biology
Author(s): Björn Kemper; Martin Götte; Burkhard Greve; Steffi Ketelhut
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Paper Abstract

Quantitative phase imaging (QPI) techniques provide high resolution label-free quantitative live cell imaging. Here, prospects and challenges of QPI in tumor cell biology are presented, using the example of digital holographic microscopy (DHM). It is shown that the evaluation of quantitative DHM phase images allows the retrieval of different parameter sets for quantification of cellular motion changes in migration and motility assays that are caused by genetic modifications. Furthermore, we demonstrate simultaneously label-free imaging of cell growth and morphology properties.

Paper Details

Date Published: 9 March 2016
PDF: 7 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97180N (9 March 2016); doi: 10.1117/12.2213488
Show Author Affiliations
Björn Kemper, Biodemical Technology Ctr., Univ. of Muenster (Germany)
Martin Götte, Muenster Univ. Hospital (Germany)
Burkhard Greve, Muenster Univ. Hospital (Germany)
Steffi Ketelhut, Biodemical Technology Ctr., Univ. of Muenster (Germany)


Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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