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Proceedings Paper

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases
Author(s): P. Marquet; K. Rothenfusser; B. Rappaz; C. Depeursinge; P. Jourdain; P. J. Magistretti
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Paper Abstract

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation of a light wave when transmitted through the observed cells, namely the quantitative phase signal (QPS), is sensitive to both cellular thickness and intracellular refractive index related to the cellular content, its accurate analysis allows to derive various cell parameters and monitor specific cell processes, which are very likely to identify new cell biomarkers. Specifically, quantitative phase-digital holographic microscopy (QP-DHM), thanks to its numerical flexibility facilitating parallelization and automation processes, represents an appealing imaging modality to both identify original cellular biomarkers of diseases as well to explore the underlying pathophysiological processes.

Paper Details

Date Published: 2 May 2016
PDF: 8 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97180K (2 May 2016); doi: 10.1117/12.2213454
Show Author Affiliations
P. Marquet, Institut Univ. en Santé Mentale de Québec, Univ. Laval (Canada)
Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)
Brain and Mind Institute, École Polytechnique Fédérale de Lausanne (Switzerland)
K. Rothenfusser, Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)
Brain Mind Institute, École Polytechnique Fédérale de Lausanne (Switzerland)
B. Rappaz, Biomolecular Screening Facility, École Polytechnique Fédérale de Lausanne (Switzerland)
C. Depeursinge, Brain Mind Institute, École Polytechnique Fédérale de Lausanne (Switzerland)
King Abdullah Univ. of Science and Technology (Saudi Arabia)
P. Jourdain, Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)
Brain Mind Institute, École Polytechnique Fédérale de Lausanne (Switzerland)
P. J. Magistretti, Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)
Brain Mind Institute, École Polytechnique Fédérale de Lausanne (Switzerland)
King Abdullah Univ. of Science and Technology (Saudi Arabia)


Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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