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Proceedings Paper

Time evolution of trapped single cell microorganism
Author(s): Silvie Bernatova; Ota Samek; Stanislav Obruca; Mojmir Sery; Pavel Zemanek; Ivana Marova
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Paper Abstract

The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way.

Paper Details

Date Published: 6 April 2016
PDF: 5 pages
Proc. SPIE 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 971104 (6 April 2016); doi: 10.1117/12.2213264
Show Author Affiliations
Silvie Bernatova, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Ota Samek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Stanislav Obruca, Brno Univ. of Technology (Czech Republic)
Mojmir Sery, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Ivana Marova, Brno Univ. of Technology (Czech Republic)


Published in SPIE Proceedings Vol. 9711:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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