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Proceedings Paper

Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis
Author(s): Po-Jung Huang; Sina Baghbani Kordmahale; Chao-Kai Chou; Hirohito Yamaguchi; Mien-Chie Hung; Jun Kameoka
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Paper Abstract

Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. “microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)”we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.

Paper Details

Date Published: 21 March 2016
PDF: 5 pages
Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970511 (21 March 2016); doi: 10.1117/12.2213259
Show Author Affiliations
Po-Jung Huang, Texas A&M Univ. (United States)
Sina Baghbani Kordmahale, Texas A&M Univ. (United States)
Chao-Kai Chou, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Hirohito Yamaguchi, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Mien-Chie Hung, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Jun Kameoka, Texas A&M Univ. (United States)
The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)

Published in SPIE Proceedings Vol. 9705:
Microfluidics, BioMEMS, and Medical Microsystems XIV
Bonnie L. Gray; Holger Becker, Editor(s)

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