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Proceedings Paper

Fluorescent scanning laser ophthalmoscopy for cellular resolution in vivo mouse retinal imaging: benefits and drawbacks of implementing adaptive optics
Author(s): Pengfei Zhang; Mayank Goswami; Edward N. Pugh; Robert J. Zawadzki
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Paper Abstract

Scanning Laser Ophthalmoscopy (SLO) utilizes the eye’s cornea and lens as the imaging objective. Thus, in a perfect eye the Numerical Aperture (NA) defined by the eye’s fully dilated pupil would limit the resolution that can be achieved. However, the eye’s ocular aberrations “blur the image”, thereby limiting the pupil size and NA for aberrationless SLO imaging. By combining SLO with Adaptive Optics (AO), AO-SLO systems can correct for ocular aberrations even over large pupil sizes and allow diffraction limited resolution in both axial and lateral directions. Here, we evaluate the effects of ocular aberrations on the performance of mouse retinal imaging with SLO and AO-SLO systems. To achieve this we first measured the RMS error of the wavefront aberrations in two populations of mice of different ages. Then, we simulated the imaging PSF and resulting lateral resolution that could be achieved with varied input beam diameter (NA) at the mouse pupil, assuming the presence of ocular aberrations (simulation of SLO imaging) and no aberrations (simulation of perfect AO-SLO imaging). The SLO system performance along with different imaging beam sizes was further assessed by computing the PSF Strehl Ratio. Finally, the advantages and limitations of SLO and AO-SLO retinal imaging in mice are discussed based on presented results.

Paper Details

Date Published: 26 April 2016
PDF: 9 pages
Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96930E (26 April 2016); doi: 10.1117/12.2213257
Show Author Affiliations
Pengfei Zhang, Univ. of California, Davis (United States)
Mayank Goswami, Univ. of California, Davis (United States)
Edward N. Pugh, UC Davis Medical Ctr. (United States)
Robert J. Zawadzki, Univ. of California, Davis (United States)
UC Davis Eye Ctr. (United States)

Published in SPIE Proceedings Vol. 9693:
Ophthalmic Technologies XXVI
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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