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Proceedings Paper

Evaluation of PpIX formation in Cervical Intraepithelial Neoplasia I (CIN) using widefield fluorescence images
Author(s): Fernanda M. Carbinatto; Natalia Mayumi Inada; Thereza C. Fortunato; Welington Lombardi; Eduardo Venerando da Silva; José Dirceu Vollet Filho; Cristina Kurachi; Sebastião Pratavieira; Vanderlei Salvador Bagnato
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Paper Abstract

Optical techniques has been described as auxiliary technology for screening of neoplasia because shows the potential for tissues differentiation in real-time and it is a noninvasive detection and safe. However, only endogenous fluorophores presents the lesion may be insufficient and needed of the administration of the fluorophores synthesized, such as, precursor molecule of protoporphyrin IX (PpIX) induced by 5- aminolevulinic acid and your derivatives. Topical application of methylaminolevulinate (MAL), induces formation of the endogenous photosensitizer, PpIX in tissues where carcinogenesis has begun. The PpIX tend to accumulate in premalignant and malignant tissues and the illumination with light with appropriate wavelength beginning to excitation of PpIX fluorescence, which helps to localize PpIX-rich areas and identify potentially malignant tissues. The aim of the study is to evaluate the production of PpIX in the cervix with CIN I through of the fluorescence images captured after 1 hour of cream application. It was possible to visualize PpIX fluorescence in cervix and it was possible to observe the selectivity in fluorescence in squamous-columnar junction, which a pre-cancerous condition (CIN) and usually is localized. Through the image processing it was possible to quantify the increase of red fluorescence. For the CIN I the increase of red fluorescence was approximately of 4 times indicating a good PpIX formation.

Paper Details

Date Published: 7 March 2016
PDF: 6 pages
Proc. SPIE 9699, Optics and Biophotonics in Low-Resource Settings II, 96990Z (7 March 2016); doi: 10.1117/12.2213250
Show Author Affiliations
Fernanda M. Carbinatto, Univ. of São Paulo (Brazil)
Natalia Mayumi Inada, Univ. of São Paulo (Brazil)
Thereza C. Fortunato, Univ. of São Paulo (Brazil)
Welington Lombardi, UNIARA (Brazil)
Eduardo Venerando da Silva, UNIARA (Brazil)
José Dirceu Vollet Filho, Univ. of São Paulo (Brazil)
Cristina Kurachi, Univ. of São Paulo (Brazil)
Sebastião Pratavieira, Univ. of São Paulo (Brazil)
Vanderlei Salvador Bagnato, Univ. of São Paulo (Brazil)

Published in SPIE Proceedings Vol. 9699:
Optics and Biophotonics in Low-Resource Settings II
David Levitz; Aydogan Ozcan; David Erickson, Editor(s)

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