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Proceedings Paper

Real-time quantum cascade laser-based infrared microspectroscopy in-vivo
Author(s): N. Kröger-Lui; K. Haase; A. Pucci; A. Schönhals; W. Petrich
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Paper Abstract

Infrared microscopy can be performed to observe dynamic processes on a microscopic scale. Fourier-transform infrared spectroscopy-based microscopes are bound to limitations regarding time resolution, which hampers their potential for imaging fast moving systems. In this manuscript we present a quantum cascade laser-based infrared microscope which overcomes these limitations and readily achieves standard video frame rates. The capabilities of our setup are demonstrated by observing dynamical processes at their specific time scales: fermentation, slow moving Amoeba Proteus and fast moving Caenorhabditis elegans. Mid-infrared sampling rates between 30 min and 20 ms are demonstrated.

Paper Details

Date Published: 7 March 2016
PDF: 8 pages
Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 97040J (7 March 2016); doi: 10.1117/12.2213239
Show Author Affiliations
N. Kröger-Lui, Kirchhoff-Institute for Physics (Germany)
K. Haase, Kirchhoff-Institute for Physics (Germany)
A. Pucci, Kirchhoff-Institute for Physics (Germany)
A. Schönhals, Kirchhoff-Institute for Physics (Germany)
W. Petrich, Kirchhoff-Institute for Physics (Germany)

Published in SPIE Proceedings Vol. 9704:
Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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