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Proceedings Paper

Differentiation of cancerous and normal brain tissue using label free fluorescence and Stokes shift spectroscopy
Author(s): Yan Zhou; Leana Wang; Cheng-hui Liu; Yong He; Xinguang Yu; Gangge Cheng; Peng Wang; Cheng Shu; Robert R. Alfano
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Paper Abstract

In this report, optical biopsy was applied to diagnose human brain cancer in vitro for the identification of brain cancer from normal tissues by native fluorescence and Stokes shift spectra (SSS). 77 brain specimens including three types of human brain tissues (normal, glioma and brain metastasis of lung cancers) were studied. In order to observe spectral changes of fluorophores via fluorescence, the selected excitation wavelength of UV at 300 and 340 nm for emission spectra and a different Stokes Shift spectra with intervals Δλ = 40 nm were measured. The fluorescence spectra and SSS from multiple key native molecular markers, such as tryptophan, collagen, NADH, alanine, ceroid and lipofuscin were observed in normal and diseased brain tissues. Two diagnostic criteria were established based on the ratios of the peak intensities and peak position in both fluorescence and SSS spectra. It was observed that the ratio of the spectral peak intensity of tryptophan (340 nm) to NADH (440 nm) increased in glioma, meningioma (benign), malignant meninges tumor, and brain metastasis of lung cancer tissues in comparison with normal tissues. The ratio of the SS spectral peak (Δλ = 40 nm) intensities from 292 nm to 366 nm had risen similarly in all grades of tumors.

Paper Details

Date Published: 7 March 2016
PDF: 6 pages
Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 97030Q (7 March 2016); doi: 10.1117/12.2213120
Show Author Affiliations
Yan Zhou, The Air Force General Hospital (China)
Leana Wang, Columbia Univ. (United States)
Cheng-hui Liu, The City College of New York (United States)
Yong He, Beijing Normal Univ. (China)
Xinguang Yu, The PLA General Hospital (China)
Gangge Cheng, The PLA General Hospital (China)
Peng Wang, The PLA General Hospital (China)
Cheng Shu, The PLA General Hospital (China)
Robert R. Alfano, The City College of New York (United States)

Published in SPIE Proceedings Vol. 9703:
Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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