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Proceedings Paper

Point spread function engineering with multiphoton SPIFI
Author(s): Keith A. Wernsing; Jeffrey J. Field; Scott R. Domingue; Alyssa M. Allende-Motz; Keith F. DeLuca; Dean H. Levi; Jennifer G. DeLuca; Michael D. Young; Jeff A. Squier; Randy A. Bartels
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Paper Abstract

MultiPhoton SPatIal Frequency modulated Imaging (MP-SPIFI) has recently demonstrated the ability to simultaneously obtain super-resolved images in both coherent and incoherent scattering processes — namely, second harmonic generation and two-photon fluorescence, respectively.1 In our previous analysis, we considered image formation produced by the zero and first diffracted orders from the SPIFI modulator. However, the modulator is a binary amplitude mask, and therefore produces multiple diffracted orders. In this work, we extend our analysis to image formation in the presence of higher diffracted orders. We find that tuning the mask duty cycle offers a measure of control over the shape of super-resolved point spread functions in an MP-SPIFI microscope.

Paper Details

Date Published: 9 March 2016
PDF: 10 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971304 (9 March 2016); doi: 10.1117/12.2213090
Show Author Affiliations
Keith A. Wernsing, Colorado State Univ. (United States)
Jeffrey J. Field, Institute for Genome Architecture and Function, Colorado State Univ. (United States)
Scott R. Domingue, Colorado State Univ. (United States)
Alyssa M. Allende-Motz, Colorado School of Mines (United States)
National Renewable Energy Lab. (United States)
Keith F. DeLuca, Colorado State Univ. (United States)
Dean H. Levi, National Renewable Energy Lab. (United States)
Jennifer G. DeLuca, Institute for Genome Architecture and Function, Colorado State Univ. (United States)
Michael D. Young, Colorado School of Mines (United States)
Jeff A. Squier, Colorado School of Mines (United States)
Randy A. Bartels, Institute for Genome Architecture and Function, Colorado State Univ. (United States)


Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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