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Proceedings Paper

An unsupervised machine learning method for delineating stratum corneum in reflectance confocal microscopy stacks of human skin in vivo
Author(s): Alican Bozkurt; Kivanc Kose; Christi A. Fox; Jennifer Dy; Dana H. Brooks; Milind Rajadhyaksha
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Paper Abstract

Study of the stratum corneum (SC) in human skin is important for research in barrier structure and function, drug delivery, and water permeability of skin. The optical sectioning and high resolution of reflectance confocal microscopy (RCM) allows visual examination of SC non-invasively. Here, we present an unsupervised segmentation algorithm that can automatically delineate thickness of the SC in RCM images of human skin in-vivo. We mimic clinicians visual process by applying complex wavelet transform over non-overlapping local regions of size 16 x 16 μm called tiles, and analyze the textural changes in between consecutive tiles in axial (depth) direction. We use dual-tree complex wavelet transform to represent textural structures in each tile. This transform is almost shift-invariant, and directionally selective, which makes it highly efficient in texture representation. Using DT-CWT, we decompose each tile into 6 directional sub-bands with orientations in ±15, 45, and 75 degrees and a low-pass band, which is the decimated version of the input. We apply 3 scales of decomposition by recursively transforming the low-pass bands and obtain 18 bands of different directionality at different scales. We then calculate mean and variance of each band resulting in a feature vector of 36 entries. Feature vectors obtained for each stack of tiles in axial direction are then clustered using spectral clustering in order to detect the textural changes in depth direction. Testing on a set of 15 RCM stacks produced a mean error of 5.45±1.32 μm, compared to the ”ground truth” segmentation provided by a clinical expert reader.

Paper Details

Date Published: 29 February 2016
PDF: 8 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96890Z (29 February 2016); doi: 10.1117/12.2213036
Show Author Affiliations
Alican Bozkurt, Northeastern Univ. (United States)
Kivanc Kose, Memorial Sloan-Kettering Cancer Ctr. (United States)
Christi A. Fox, Caliber Imaging and Diagnostics, Inc. (United States)
Jennifer Dy, Northeastern Univ. (United States)
Dana H. Brooks, Northeastern Univ. (United States)
Milind Rajadhyaksha, Memorial Sloan-Kettering Cancer Ctr. (United States)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong; Justus F. Ilgner; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory; Laura Marcu; Haishan Zeng, Editor(s)

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