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Proceedings Paper

A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice
Author(s): Karina S. Litvinova; Shakil Ahmad; Keqing Wang; Ilya E. Rafailov; Sergei G. Sokolovski; Lin Zhang; Edik U. Rafailov; Asif Ahmed
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Paper Abstract

Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

Paper Details

Date Published: 29 February 2016
PDF: 8 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96893H (29 February 2016); doi: 10.1117/12.2213026
Show Author Affiliations
Karina S. Litvinova, Aston Institute of Photonic Technologies (United Kingdom)
Shakil Ahmad, Aston Medical Research Institute (United Kingdom)
Keqing Wang, Aston Medical Research Institute (United Kingdom)
Ilya E. Rafailov, Aston Institute of Photonic Technologies (United Kingdom)
Sergei G. Sokolovski, Aston Institute of Photonic Technologies (United Kingdom)
Lin Zhang, Aston Institute of Photonic Technologies (United Kingdom)
Edik U. Rafailov, Aston Institute of Photonic Technologies (United Kingdom)
Asif Ahmed, Aston Medical Research Institute (United Kingdom)


Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong; Justus F. Ilgner; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory; Laura Marcu; Haishan Zeng, Editor(s)

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