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Proceedings Paper

Nonlinear optical Stokes ellipsometric (NOSE) microscopy for imaging the nonlinear susceptibility tensors of collagen
Author(s): Ximeng Y. Dow; Emma L. DeWalt; Shane Z. Sullivan; Paul D. Schmitt; Garth J. Simpson
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Paper Abstract

Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen structure in a mouse tail section. NOSE is based on polarization-dependent second harmonic generation (SHG) imaging. The fast polarization-modulation was achieved using an electro-optic modulator (EOM), allowing for the potential of video-rate NOSE analysis. The signal to noise advantages associated with suppression of 1/f noise by rapid polarization modulation allowed reliable recovery of the local-frame tensor on a per-pixel basis. An iterative approach involving laboratory to local frame coordinate transformation was developed to recover the spatial distribution of local-frame nonlinear susceptibility tensor elements of collagen as well as the polar and azimuthal orientation angles of the collagen structure.

Paper Details

Date Published: 14 March 2016
PDF: 7 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97121S (14 March 2016); doi: 10.1117/12.2212944
Show Author Affiliations
Ximeng Y. Dow, Purdue Univ. (United States)
Emma L. DeWalt, Purdue Univ. (United States)
Shane Z. Sullivan, Purdue Univ. (United States)
Paul D. Schmitt, Purdue Univ. (United States)
Garth J. Simpson, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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