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Proceedings Paper

Chronic monitoring of cortical hemodynamics in behaving, freely-moving rats using a miniaturized head-mounted optical microscope
Author(s): Iliya Sigal; Raanan Gad; Margaret Koletar; Dene Ringuette; Bojana Stefanovic; Ofer Levi
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Paper Abstract

Growing interest within the neurophysiology community in assessing healthy and pathological brain activity in animals that are awake and freely-behaving has triggered the need for optical systems that are suitable for such longitudinal studies. In this work we report label-free multi-modal imaging of cortical hemodynamics in the somatosensory cortex of awake, freely-behaving rats, using a novel head-mounted miniature optical microscope. The microscope employs vertical cavity surface emitting lasers (VCSELs) at three distinct wavelengths (680 nm, 795 nm, and 850 nm) to provide measurements of four hemodynamic markers: blood flow speeds, HbO, HbR, and total Hb concentration, across a > 2 mm field of view. Blood flow speeds are extracted using Laser Speckle Contrast Imaging (LSCI), while oxygenation measurements are performed using Intrinsic Optical Signal Imaging (IOSI). Longitudinal measurements on the same animal are made possible over the course of > 6 weeks using a chronic window that is surgically implanted into the skull. We use the device to examine changes in blood flow and blood oxygenation in superficial cortical blood vessels and tissue in response to drug-induced absence-like seizures, correlating motor behavior with changes in blood flow and blood oxygenation in the brain.

Paper Details

Date Published: 9 March 2016
PDF: 5 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 969010 (9 March 2016); doi: 10.1117/12.2212936
Show Author Affiliations
Iliya Sigal, Univ. of Toronto (Canada)
Raanan Gad, Univ. of Toronto (Canada)
Margaret Koletar, Sunnybrook Research Institute (Canada)
Dene Ringuette, Univ. of Toronto (Canada)
Bojana Stefanovic, Sunnybrook Research Institute (Canada)
Ofer Levi, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; Victor X. D. Yang; E. Duco Jansen; Qingming Luo; Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)

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