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Proceedings Paper

Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules
Author(s): Wonju Lee; Youngjin Oh; Kyujung Kim; Yoshiaki Kinosita; Nagisa Mikami; Takayuki Nishizaka; Donghyun Kim
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Paper Abstract

In this presentation, we explore the feasibility of plasmonic nanohole-based sub-diffraction-limited nanoscopy for biomolecular imaging. The technique utilizes near-field distribution localized by surface plasmon localization on metallic nanoholes which is used to sample molecular fluorescence. The optimum geometry of nanohole arrays was determined by numerical analysis. The localization sampling was applied to reconstructing sub-diffraction-limited images of gliding microtubules with a 76 nm effective resolution in the lateral direction. Extraordinary light transmission was also employed to address enhancement of axial resolution using nanohole arrays, based on which extraction of gliding motions of bacteria was demonstrated with an axial resolution down to 50 nm.

Paper Details

Date Published: 22 April 2016
PDF: 7 pages
Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 972107 (22 April 2016); doi: 10.1117/12.2212903
Show Author Affiliations
Wonju Lee, Yonsei Univ. (Korea, Republic of)
Youngjin Oh, Yonsei Univ. (Korea, Republic of)
Kyujung Kim, Pusan National Univ. (Korea, Republic of)
Yoshiaki Kinosita, Gakushuin Univ. (Japan)
Nagisa Mikami, Gakushuin Univ. (Japan)
Takayuki Nishizaka, Gakushuin Univ. (Japan)
Donghyun Kim, Yonsei Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 9721:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Alexander N. Cartwright; Dan V. Nicolau; Dror Fixler, Editor(s)

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