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Proceedings Paper

Selective-plane illumination microscopy for high-content volumetric biological imaging
Author(s): Ryan McGorty; Bo Huang
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Paper Abstract

Light-sheet microscopy, also named selective-plane illumination microscopy, enables optical sectioning with minimal light delivered to the sample. Therefore, it allows one to gather volumetric datasets of developing embryos and other light-sensitive samples over extended times. We have configured a light-sheet microscope that, unlike most previous designs, can image samples in formats compatible with high-content imaging. Our microscope can be used with multi-well plates or with microfluidic devices. In designing our optical system to accommodate these types of sample holders we encounter large optical aberrations. We counter these aberrations with both static optical components in the imaging path and with adaptive optics. Potential applications of this microscope include studying the development of a large number of embryos in parallel and over long times with subcellular resolution and doing high-throughput screens on organisms or cells where volumetric data is necessary.

Paper Details

Date Published: 7 March 2016
PDF: 8 pages
Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200P (7 March 2016); doi: 10.1117/12.2212889
Show Author Affiliations
Ryan McGorty, Univ. of San Diego (United States)
Univ. of California, San Francisco (United States)
Bo Huang, Univ. of California, San Francisco (United States)


Published in SPIE Proceedings Vol. 9720:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management
Kevin K. Tsia; Keisuke Goda, Editor(s)

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