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Proceedings Paper

Optical metabolic imaging for monitoring tracheal health
Author(s): Joe T. Sharick; Daniel A. Gil; Michael A. Choma; Melissa C. Skala
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Paper Abstract

The health of the tracheal mucosa and submucosa is a vital yet poorly understood component of critical care medicine, and a minimally-invasive method is needed to monitor tracheal health in patients. Of particular interest are the ciliated cells of the tracheal epithelium that move mucus away from the lungs and prevent respiratory infection. Optical metabolic imaging (OMI) allows cellular-level measurement of metabolism, and is a compelling method for assessing tracheal health because ciliary motor proteins require ATP to function. In this pilot study, we apply multiphoton imaging of the fluorescence intensities and lifetimes of metabolic co-enzymes NAD(P)H and FAD to the mucosa and submucosa of ex vivo mouse trachea. We demonstrate the feasibility and potential diagnostic utility of these measurements for assessing tracheal health and pathophysiology at the single-cell level.

Paper Details

Date Published: 6 April 2016
PDF: 5 pages
Proc. SPIE 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 971108 (6 April 2016); doi: 10.1117/12.2212779
Show Author Affiliations
Joe T. Sharick, Vanderbilt Univ. (United States)
Daniel A. Gil, Vanderbilt Univ. (United States)
Michael A. Choma, Yale Univ. (United States)
Melissa C. Skala, Vanderbilt Univ. (United States)


Published in SPIE Proceedings Vol. 9711:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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