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Proceedings Paper

The integration of single fiber reflectance (SFR) spectroscopy during endoscopic ultrasound-guided fine needle aspirations (EUS-FNA) in pancreatic masses: a feasibility study
Author(s): Paulien L. Stegehuis; Leonora S. F. Boogerd; Akin Inderson; Roeland A. Veenendaal; Bert A. Bonsing; Arjen Amelink; Alexander L. Vahrmeijer; Jouke Dijkstra; Dominic J. Robinson
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Paper Abstract

EUS-FNA can be used for pathological confirmation of a suspicious pancreatic mass. However, performance depends on an on-site cytologist and time between punction and final pathology results can be long. SFR spectroscopy is capable of extracting biologically relevant parameters (e.g. oxygenation and blood volume) in real-time from a very small tissue volume at difficult locations. In this study we determined feasibility of the integration of SFR spectroscopy during EUSFNA procedures in pancreatic masses. Patients with benign and malignant pancreatic masses who were scheduled for an EUS-FNA were included. The working guide wire inside the 19 gauge endoscopic biopsy needle was removed and the sterile single fiber (300 μm core and 700 μm outer diameter, wide-angle beam, NA 0.22) inserted through the needle. Spectroscopy measurements in the visiblenear infrared wavelength region (400-900 nm) and autofluorescence measurements (excitation at 405 nm) were taken three times, and subsequently cytology was obtained. Wavelength dependent optical properties were compared to cytology results. We took measurements in 13 patients with corresponding cytology results (including mucinous tumor, ductal adenocarcinoma, neuroendocrine tumor, and pancreatitis). In this paper we show the first analyzed results comparing normal pancreatic tissue with cancerous tissue in the same patient. We found a large difference in blood volume fraction, and blood oxygenation was higher in normal tissue. Integration of SFR spectroscopy is feasible in EUS-FNA procedures, the workflow hardly requires changes and it takes little time. The first results differentiating normal from tumor tissue are promising.

Paper Details

Date Published: 8 March 2016
PDF: 6 pages
Proc. SPIE 9691, Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III, 96910J (8 March 2016); doi: 10.1117/12.2212623
Show Author Affiliations
Paulien L. Stegehuis, Leiden Univ. Medical Ctr. (Netherlands)
Leonora S. F. Boogerd, Leiden Univ. Medical Ctr. (Netherlands)
Akin Inderson, Leiden Univ. Medical Ctr (Netherlands)
Roeland A. Veenendaal, Leiden Univ. Medical Ctr. (Netherlands)
Bert A. Bonsing, Leiden Univ. Medical Ctr. (Netherlands)
Arjen Amelink, TNO (Netherlands)
Alexander L. Vahrmeijer, Leiden Univ. Medical Ctr. (Netherlands)
Jouke Dijkstra, Leiden Univ. Medical Ctr. (Netherlands)
Dominic J. Robinson, Erasmus Medical Ctr. (Netherlands)


Published in SPIE Proceedings Vol. 9691:
Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III
Melissa J. Suter; Guillermo J. Tearney; Thomas D. Wang; Stephen Lam; Matthew Brenner, Editor(s)

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