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Proceedings Paper

Investigate the variation in optical redox ratio of epicardial adipose tissue in patients with CAD through auto-fluorescence metabolic molecular image (Conference Presentation)
Author(s): Lun-Zhang Guo; Tzung-Dau Wang; Jong-Wei Lin; Tzu-Ming Liu

Paper Abstract

In recent years, it has been suggested that epicardial adipose tissue (EAT) plays an important role in development of coronary artery disease (CAD) and diabetes mellitus (DM). In this article, we used two-photon fluoresce microscope to measure the fluorescence metabolic image of EAT, which obtained from the patient with/without CAD/DM. We used 740nm and 890nm infrared light to excite the auto-fluorescence of metabolic molecules NADH and FAD respectively. We collected the fluorescence signal at wavelength 450nm to 500nm and 500nm to 550nm to obtain the metabolic image. Through the image, we computed the redox ratio (NADH/FAD) by analyzing the intensity. The preliminary result showed that the redox ratio increase in the patients with CAD. It indicates EAT adipocytes of patient with CAD have decreased cellular metabolic activity. But there were no significant variation of redox ratio in the patients with DM.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 971114 (27 April 2016); doi: 10.1117/12.2212606
Show Author Affiliations
Lun-Zhang Guo, National Taiwan Univ. (Taiwan)
Tzung-Dau Wang, Cardiovascular Ctr., National Taiwan Univ. Hospital (Taiwan)
Molecular Imaging Ctr., National Taiwan Univ. (Taiwan)
Jong-Wei Lin, Cardiovascular Ctr., National Taiwan Univ. Hospital (Taiwan)
Tzu-Ming Liu, Institute of Biomedical Engineering, National Taiwan Univ. (Taiwan)
Molecular Imaging Ctr., National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 9711:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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