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Proceedings Paper

Volumetric real-time wide-field microscopy with tunable acoustic gradient lens (Conference Presentation)
Author(s): Ting Hsuan Chen; Craig B. Arnold
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Paper Abstract

Three-dimensional real-time imaging is a fundamental challenge for understanding dynamic processes. In this presentation, we propose a new method based on a tunable acoustic gradient lens integrated in a simple optical system. By synchronizing a pulsed LED with a high-speed camera, we are able to resolve a volume of 2 millimeters and 2 millimeters with depth 1 millimeter in 7 microseconds. A simulation model of the optical system is provided and serves as a useful tool for designing the optical system for the desired aspect-ratio of the imaging volume. The ability to resolve a volume in microseconds opens the door to exploring the fundamental dynamics in micro scale.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971307 (27 April 2016); doi: 10.1117/12.2212604
Show Author Affiliations
Ting Hsuan Chen, Princeton Univ. (United States)
Craig B. Arnold, Princeton Univ. (United States)

Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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