Share Email Print
cover

Proceedings Paper

Image-guided cold atmosphere plasma (CAP) therapy for cutaneous wound
Author(s): Zelin Yu; Wenqi Ren; Qi Gan; Jiahong Li; XiangXiang Li; Shiwu Zhang; Fan Jin; Cheng Cheng; Yue Ting; Ronald X. Xu
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Bacterial infection is one of the major factors contributing to the compromised healing in chronic wounds. Sometimes bacteria biofilms formed on the wound are more resistant than adherent bacteria. Cold atmosphere plasma (CAP) has already shown its potential in contact-free disinfection, blood coagulation, and wound healing. In this study, we integrated a multimodal imaging system with a portable CAP device for image-guided treatment of infected wound in vivo and evaluated the antimicrobial effect on Pseudomonas aeruginosa sample in vitro.15 ICR mice were divided into three groups for therapeutic experiments:(1) control group with no infection nor treatment (2) infection group without treatment (3) infection group with treatment. For each mouse, a three millimeters punch biopsy was created on the dorsal skin. Infection was induced by Staphylococcus aureus inoculation one day post-wounding. The treated group was subjected to CAP for 2 min daily till day 13. For each group, five fixed wounds’ oxygenation and blood perfusion were evaluated daily till day 13 by a multimodal imaging system that integrates a multispectral imaging module and a laser speckle imaging module. In the research of relationship between therapeutic depth and sterilization effect on P.aeruginosa in agarose, we found that the CAP-generated reactive species reached the depth of 26.7μm at 30s and 41.6μm at 60s for anti-bacterial effects. Image-guided CAP therapy can be potentially used to control infection and facilitate the healing process of infected wounds.

Paper Details

Date Published: 7 March 2016
PDF: 13 pages
Proc. SPIE 9698, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV, 96980K (7 March 2016); doi: 10.1117/12.2212516
Show Author Affiliations
Zelin Yu, Univ. of Science and Technology of China (China)
Wenqi Ren, Univ. of Science and Technology of China (China)
The Ohio State Univ. (United States)
Qi Gan, Univ. of Science and Technology of China (China)
Jiahong Li, National Lab. for Physical Sciences at the Microscale (China)
XiangXiang Li, Univ. of Science and Technology of China (China)
Shiwu Zhang, Univ. of Science and Technology of China (China)
Fan Jin, National Lab. for Physical Sciences at the Microscale (China)
Cheng Cheng, Institute of Plasma Physics (China)
Yue Ting, Univ. of Science and Technology of China (China)
Ronald X. Xu, Univ. of Science and Technology of China (China)
The Ohio State Univ. (United States)


Published in SPIE Proceedings Vol. 9698:
Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

© SPIE. Terms of Use
Back to Top