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Proceedings Paper

Evaluation of free radical scavenging capacity and antioxidative damage effect of resveratrol-nanostructured lipid carriers
Author(s): Ju Jin; Fan Shi; Qiu-wen Li; Pei-shan Li; Tong-sheng Chen; Yi-fei Wang; Zhi-ping Wang
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Paper Abstract

Cellular damage induced by free-radicals like reactive oxygen species has been implicated in several diseases. 2, 2-azobis(2-amidino-propane) dihydrochloride(AAPH) generates two potent ROS capable of inducing lipid peroxidation: alkoxy radical(RO-) and peroxy radical(ROO-). These radicals are similar to those that are physiologically active and thus might initiate a cascade of intracellular toxic events leading to oxidation, lipid peroxidation, DNA damage and subsequent cell death. Hence naturally anti-oxidant play a vital role in combating these conditions. In this study, resveratrol loaded nanostructured lipid carriers (Res-NLC) was prepared by hot melting and then high pressure homogenization technique. The effects of Res-NLC on free radical scavenging capacity and antioxidative damage is investigated. The particle size and zeta potential of Res-NLC were 139.3 ± 1.7 nm and −11.21 ± 0.41 mV, respectively. By free radical scavenging assays, the IC50 value of Res-NLC were 19.25, 5.29 μg/mL with DPPH, ABTS assay respectively, and 0.161 mg ferrous sulfate/1 mg Res-NLC with FRAP assay; and by AAPH-induced oxidative injury cell model assay, Res-NLC showed the strong protective effect against the human liver tumor HepG2 cell oxidative stress damage. These results indicated that the antioxidant properties of Res-NLC hold great potential used as an alternative to more toxic synthetic antioxidants as an additive in food, cosmetic and pharmaceutical preparations for the oxidative diseases treatment.

Paper Details

Date Published: 22 April 2016
PDF: 7 pages
Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97221D (22 April 2016); doi: 10.1117/12.2212464
Show Author Affiliations
Ju Jin, Guangdong Pharmaceutical Univ. (China)
Fan Shi, Guangdong Pharmaceutical Univ. (China)
Qiu-wen Li, Guangdong Pharmaceutical Univ. (China)
Pei-shan Li, Guangdong Pharmaceutical Univ. (China)
Tong-sheng Chen, South China Normal Univ. (China)
Yi-fei Wang, Jinan Univ. (China)
Zhi-ping Wang, Guangdong Pharmaceutical Univ. (China)

Published in SPIE Proceedings Vol. 9722:
Colloidal Nanoparticles for Biomedical Applications XI
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

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