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Proceedings Paper

Quantitative phase imaging of biological cells and tissues using singleshot white light interference microscopy and phase subtraction method for extended range of measurement
Author(s): Dalip Singh Mehta; Anuradha Sharma; Vishesh Dubey; Veena Singh; Azeem Ahmad
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Paper Abstract

We present a single-shot white light interference microscopy for the quantitative phase imaging (QPI) of biological cells and tissues. A common path white light interference microscope is developed and colorful white light interferogram is recorded by three-chip color CCD camera. The recorded white light interferogram is decomposed into the red, green and blue color wavelength component interferograms and processed it to find out the RI for different color wavelengths. The decomposed interferograms are analyzed using local model fitting (LMF)" algorithm developed for reconstructing the phase map from single interferogram. LMF is slightly off-axis interferometric QPI method which is a single-shot method that employs only a single image, so it is fast and accurate. The present method is very useful for dynamic process where path-length changes at millisecond level. From the single interferogram a wavelength-dependent quantitative phase imaging of human red blood cells (RBCs) are reconstructed and refractive index is determined. The LMF algorithm is simple to implement and is efficient in computation. The results are compared with the conventional phase shifting interferometry and Hilbert transform techniques.

Paper Details

Date Published: 9 March 2016
PDF: 8 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 971828 (9 March 2016); doi: 10.1117/12.2212453
Show Author Affiliations
Dalip Singh Mehta, Indian Institute of Technology Delhi (India)
Anuradha Sharma, Indian Institute of Technology Delhi (India)
Vishesh Dubey, Indian Institute of Technology Delhi (India)
Veena Singh, Indian Institute of Technology Delhi (India)
Azeem Ahmad, Indian Institute of Technology Delhi (India)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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