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Proceedings Paper

Label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry
Author(s): Xiaoling Wang; Ping Yang; Rongrong Liu; Zhenyu Niu; Yuanzhen Suo; Hao He; Wenyuan Gao; Shuo Tang; Xunbin Wei
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Paper Abstract

Melanoma is a malignant tumor of melanocytes. Melanoma cells have high light absorption due to melanin highly contained in melanoma cells. This property is employed for the detection of circulating melanoma cell by in vivo photoacoustic flow cytometry (PAFC), which is based on photoacoustic effect. Compared to in vivo flow cytometry based on fluorescence, PAFC can employ high melanin content of melanoma cells as endogenous biomarkers to detect circulating melanoma cells in vivo. We have developed in vitro experiments to prove the ability of PAFC system of detecting photoacoustic signals from melanoma cells. For in vivo experiments, we have constructed a model of melanoma tumor bearing mice by inoculating highly metastatic murine melanoma cancer cells, B16F10 with subcutaneous injection. PA signals are detected in the blood vessels of mouse ears in vivo. The raw signal detected from target cells often contains some noise caused by electronic devices, such as background noise and thermal noise. We choose the Wavelet denoising method to effectively distinguish the target signal from background noise. Processing in time domain and frequency domain would be combined to analyze the signal after denoising. This algorithm contains time domain filter and frequency transformation. The frequency spectrum image of the signal contains distinctive features that can be used to analyze the property of target cells or particles. The processing methods have a great potential for analyzing signals accurately and rapidly. By counting circulating melanoma cells termly, we obtain the number variation of circulating melanoma cells as melanoma metastasized. Those results show that PAFC is a noninvasive and label-free method to detect melanoma metastases in blood or lymph circulation.

Paper Details

Date Published: 9 March 2016
PDF: 5 pages
Proc. SPIE 9709, Biophotonics and Immune Responses XI, 97090C (9 March 2016); doi: 10.1117/12.2212234
Show Author Affiliations
Xiaoling Wang, Shanghai Jiao Tong Univ. (China)
Ping Yang, Shanghai Jiao Tong Univ. (China)
Rongrong Liu, Shanghai Jiao Tong Univ. (China)
Zhenyu Niu, Shanghai Jiao Tong Univ. (China)
Yuanzhen Suo, Shanghai Jiao Tong Univ. (China)
Hao He, Shanghai Jiao Tong Univ. (China)
Wenyuan Gao, Binzhou Medical Univ. (China)
Shuo Tang, Univ. of British Columbia (Canada)
Xunbin Wei, Shanghai Jiao Tong Univ. (China)


Published in SPIE Proceedings Vol. 9709:
Biophotonics and Immune Responses XI
Wei R. Chen, Editor(s)

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