Share Email Print

Proceedings Paper

In vivo switchable optical- and acoustic-resolution photoacoustic microscopy
Author(s): Seungwan Jeon; Jaewoo Kim; Chulhong Kim
Format Member Price Non-Member Price
PDF $17.00 $21.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Photoacoustic microscopy (PAM) provides high resolution and large penetration depth by utilizing the high optical sensitivity and low scattering of ultrasound. Hybrid PAM systems can be classified into two categories: opticalresolution photoacoustic microscopy (OR-PAM) and acoustic-resolution photoacoustic microscopy (AR-PAM). ORPAM provides a very high lateral resolution with a strong optical focus, but the penetration depth is limited to one optical transport mean free path. AR-PAM provides a relatively greater penetration depth using diffused light in biological tissues. The resolution of AR-PAM is determined by its ultrasonic parameters. In this study, we performed an in vivo testing of a switchable OR-/AR-PAM system. In this system, two modes can be switched by changing its collimator lens and optical fiber. The lateral resolution of OR-PAM was measured using a resolution test target, and the full width at half maximum (FWHM) of the edge spread function was 2.5 μm. To calculate the lateral resolution of ARPAM, a 6-μm-diameter carbon fiber was used, and the FWHM of the line spread function was 80.2 μm. We successfully demonstrated the multiscale imaging capability of the switchable OR-/AR-PAM system by visualizing microvascular networks in mouse ears, brain, legs, skin, and eyes.

Paper Details

Date Published: 15 March 2016
PDF: 5 pages
Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 970845 (15 March 2016); doi: 10.1117/12.2212199
Show Author Affiliations
Seungwan Jeon, Pohang Univ. of Science and Technology (Korea, Republic of)
Jaewoo Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
Chulhong Kim, Pohang Univ. of Science and Technology (Korea, Republic of)

Published in SPIE Proceedings Vol. 9708:
Photons Plus Ultrasound: Imaging and Sensing 2016
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top