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Proceedings Paper

Bowel perforation detection using metabolic fluorescent chlorophylls
Author(s): Jung Hyun Han; Young Goun Jo; Jung Chul Kim; Sujeong Choi; Hoonsoo Kang; Yong-Chul Kim; In-Wook Hwang
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Paper Abstract

Thus far, there have been tries of detection of disease using fluorescent materials. We introduce the chlorophyll derivatives from food plants, which have longer-wavelength emissions (at >650 nm) than those of fluorescence of tissues and organs, for detection of bowel perforation. To figure out the possibility of fluorescence spectroscopy as a monitoring sensor of bowel perforation, fluorescence from organs of rodent models, intestinal and peritoneal fluids of rodent models and human were analyzed. In IVIS fluorescence image of rodent abdominal organ, visualization of perforated area only was possible when threshold of image is extremely finely controlled. Generally, both perforated area of bowel and normal bowel which filled with large amount of chlorophyll derivatives were visualized with fluorescence. The fluorescence from chlorophyll derivatives penetrated through the normal bowel wall makes difficult to distinguish perforation area from normal bowel with direct visualization of fluorescence. However, intestinal fluids containing chlorophyll derivatives from food contents can leak from perforation sites in situation of bowel perforation. It may show brighter and longer-wavelength regime emissions of chlorophyll derivatives than those of pure peritoneal fluid or bioorgans. Peritoneal fluid mixed with intestinal fluids show much brighter emissions in longer wavelength (at>650 nm) than those of pure peritoneal fluid. In addition, irrigation fluid, which is used for the cleansing of organ and peritoneal cavity, made of mixed intestinal and peritoneal fluid diluted with physiologic saline also can be monitored bowel perforation during surgery.

Paper Details

Date Published: 7 March 2016
PDF: 7 pages
Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 970213 (7 March 2016); doi: 10.1117/12.2212111
Show Author Affiliations
Jung Hyun Han, Gwangju Institute of Science and Technology (Korea, Republic of)
Young Goun Jo, Chonnam National Univ. Hospital (Korea, Republic of)
Jung Chul Kim, Chonnam National Univ. Hospital (Korea, Republic of)
Sujeong Choi, Gwangju Institute of Science and Technology (Korea, Republic of)
Hoonsoo Kang, Gwangju Institute of Science and Technology (Korea, Republic of)
Yong-Chul Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
In-Wook Hwang, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 9702:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI
Israel Gannot, Editor(s)

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