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Proceedings Paper

3D imaging of the cleared intact murine colon with light sheet microscopy
Author(s): B. Zufiria; D. I. Bocancea; M. V. Gómez-Gaviro; J. J. Vaquero; M. Desco; M. Fresno; J. Ripoll; A. Arranz
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Paper Abstract

We here show 3D light sheet microscopy images of fixed and cleared murine colon tissue in-toto, which offer relevant cellular information without the need for physically sectioning the tissue. We have applied the recently developed CUBIC protocol (Susaki et al. Cell 157:726, 2014) for colon tissues and have found that this clearing protocol enables imaging all the way to the central part of the lumen with cellular resolution, thus opening new ways for 3D imaging of colon samples.

Paper Details

Date Published: 9 March 2016
PDF: 5 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97130Q (9 March 2016); doi: 10.1117/12.2212039
Show Author Affiliations
B. Zufiria, CSIC-Univ. Autónoma de Madrid (Spain)
Univ. Carlos III de Madrid (Spain)
D. I. Bocancea, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria Gregorio Marañón (Spain)
M. V. Gómez-Gaviro, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria Gregorio Marañón (Spain)
J. J. Vaquero, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria Gregorio Marañón (Spain)
M. Desco, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria Gregorio Marañón (Spain)
M. Fresno, CSIC-Univ. Autónoma de Madrid (Spain)
J. Ripoll, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria Gregorio Marañón (Spain)
A. Arranz, CSIC-Univ. Autónoma de Madrid (Spain)


Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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