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Proceedings Paper

High-resolution stimulated Raman scattering microscopy by focal-field modulation
Author(s): Jian Lin; Wei Zheng; Zhiwei Huang
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Paper Abstract

We report on a high-resolution stimulated Raman scattering (SRS) microscopy by phase modulation of the pump beam. In this study, an optimized phase pattern is applied to the pump beam by a spatial light modulator to minimize its focal spot size. Simulation shows that the central lobe of focused pump beam is reduced to around half of its original size, and the full-width at half-maximum of corresponding SRS distribution is reduced to less than 65% after focal-field modulation. We demonstrate this scheme by measuring the pump beam focal spot size and SRS imaging of PMMA beads.

Paper Details

Date Published: 14 March 2016
PDF: 5 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971220 (14 March 2016); doi: 10.1117/12.2211978
Show Author Affiliations
Jian Lin, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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