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Proceedings Paper

Quantitative phase imaging of living cells with a swept laser source
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Paper Abstract

Digital holographic phase microscopy is a well-established quantitative phase imaging technique. However, interference artifacts from inside the system, typically induced by elements whose optical thickness are within the source coherence length, limit the imaging quality as well as sensitivity. In this paper, a swept laser source based technique is presented. Spectra acquired at a number of wavelengths, after Fourier Transform, can be used to identify the sources of the interference artifacts. With proper tuning of the optical pathlength difference between sample and reference arms, it is possible to avoid these artifacts and achieve sensitivity below 0.3nm. Performance of the proposed technique is examined in live cell imaging.

Paper Details

Date Published: 9 March 2016
PDF: 5 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 971827 (9 March 2016); doi: 10.1117/12.2211931
Show Author Affiliations
Shichao Chen, Virginia Tech (United States)
Yizheng Zhu, Virginia Tech (United States)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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