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Proceedings Paper

Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging
Author(s): Lixin Liu; Artem Pliss; Xiao Peng; Andrey Kuzmin; Junle Qu; Paras N. Prasad
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Paper Abstract

Measurements and monitoring of concentrations of macromolecules in live cells and sub-cellular structures is of tremendous interest in cell biology and translational medicine. In this report we demonstrate a breakthrough potential of FLIM for real-time quantitative mapping of macromolecular distribution in the cell. In our approach we exploit a correlation existing between the fluorescence lifetime of fluorophores, refractive index and local concentrations of cellular macromolecules in the of fluorophore's microenvironment. We show a value of our approach for fundamental cell science and cellular diagnostic assays.

Paper Details

Date Published: 14 March 2016
PDF: 8 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971221 (14 March 2016); doi: 10.1117/12.2211889
Show Author Affiliations
Lixin Liu, Xidian Univ. (China)
Artem Pliss, Univ. at Buffalo, State Univ. of New York (United States)
Xiao Peng, Shenzhen Univ. (China)
Andrey Kuzmin, Univ. at Buffalo, State Univ. of New York (United States)
Junle Qu, Shenzhen Univ. (China)
Paras N. Prasad, Univ. at Buffalo, State Univ. of New York (United States)


Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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