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Proceedings Paper

3D differential phase contrast microscopy
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Paper Abstract

We demonstrate three-dimensional (3D) optical phase and amplitude reconstruction based on coded source illumination using a programmable LED array. Multiple stacks of images along the optical axis are computed from recorded intensities captured by multiple images under off-axis illumination. Based on the first Born approximation, a linear differential phase contrast (DPC) model is built between 3D complex index of refraction and the intensity stacks. Therefore, 3D volume reconstruction can be achieved via a fast inversion method, without the intermediate 2D phase retrieval step. Our system employs spatially partially coherent illumination, so the transverse resolution achieves twice the NA of coherent systems, while axial resolution is also improved 2× as compared to holographic imaging.

Paper Details

Date Published: 12 April 2016
PDF: 7 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 971826 (12 April 2016); doi: 10.1117/12.2211817
Show Author Affiliations
Michael Chen, Univ. of California, Berkeley (United States)
Lei Tian, Univ. of California, Berkeley (United States)
Laura Waller, Univ. of California, Berkeley (United States)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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