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Proceedings Paper

Unsupervised color normalisation for H and E stained histopathology image analysis
Author(s): Raúl Celis; Eduardo Romero
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Paper Abstract

In histology, each dye component attempts to specifically characterise different microscopic structures. In the case of the Hematoxylin-Eosin (H&E) stain, universally used for routine examination, quantitative analysis may often require the inspection of different morphological signatures related mainly to nuclei patterns, but also to stroma distribution. Nevertheless, computer systems for automatic diagnosis are often fraught by color variations ranging from the capturing device to the laboratory specific staining protocol and stains. This paper presents a novel colour normalisation method for H&E stained histopathology images. This method is based upon the opponent process theory and blindly estimates the best color basis for the Hematoxylin and Eosin stains without relying on prior knowledge. Stain Normalisation and Color Separation are transversal to any Framework of Histopathology Image Analysis.

Paper Details

Date Published: 22 December 2015
PDF: 7 pages
Proc. SPIE 9681, 11th International Symposium on Medical Information Processing and Analysis, 968104 (22 December 2015); doi: 10.1117/12.2211536
Show Author Affiliations
Raúl Celis, Univ. Nacional de Colombia (Colombia)
Eduardo Romero, Univ. Nacional de Colombia (Colombia)


Published in SPIE Proceedings Vol. 9681:
11th International Symposium on Medical Information Processing and Analysis
Eduardo Romero; Natasha Lepore; Juan D. García-Arteaga; Jorge Brieva, Editor(s)

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