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Proceedings Paper

Near-infrared hyperspectral microscopy of carbon nanotube photoluminescence enables 17-color imaging (Conference Presentation)
Author(s): Daniel A. Heller; Daniel Roxbury; Prakrit V. Jena; Ryan M, Williams; Balázs Enyedi; Philipp Niethammer; Stéphane Marcet; Francesca Mangiarini; Marc Verhaegen; Sébastien Blais-Ouellette

Paper Abstract

The intrinsic near-infrared photoluminescence (fluorescence) of single-walled carbon nanotubes exhibits unique photostability, narrow bandwidth, penetration through biological media, environmental sensitivity, and both chromatic variety and range. Biomedical applications exploiting this large family of fluorophores will require the spectral and spatial resolution of individual (n,m) nanotube species’ fluorescence and its modulation within live cells and tissues, which is not possible with current microscopy methods. We present a wide-field hyperspectral approach to spatially delineate and spectroscopically measure single nanotube fluorescence in living systems. This approach resolved up to 17 distinct (n,m) species (chiralities) with single nanotube spatial resolution in live mammalian cells, murine tissues ex vivo, and zebrafish endothelium in vivo. We anticipate that this approach will facilitate multiplexed nanotube imaging in biomedical applications while enabling deep-tissue optical penetration, exceptional photostability, and single-molecule resolution in vivo.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 97210N (27 April 2016); doi: 10.1117/12.2211387
Show Author Affiliations
Daniel A. Heller, Memorial Sloan-Kettering Cancer Ctr. (United States)
Weill Cornell Medical College (United States)
Daniel Roxbury, Memorial Sloan-Kettering Cancer Ctr. (United States)
Prakrit V. Jena, Memorial Sloan-Kettering Cancer Ctr. (United States)
Ryan M, Williams, Memorial Sloan-Kettering Cancer Ctr. (United States)
Balázs Enyedi, Memorial Sloan-Kettering Cancer Ctr. (United States)
Philipp Niethammer, Memorial Sloan-Kettering Cancer Ctr. (United States)
Stéphane Marcet, Photon etc. Inc. (Canada)
Francesca Mangiarini, Photon etc. Inc. (Canada)
Marc Verhaegen, Photon etc. Inc. (Canada)
Sébastien Blais-Ouellette, Photon etc. Inc. (Canada)


Published in SPIE Proceedings Vol. 9721:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Alexander N. Cartwright; Dan V. Nicolau; Dror Fixler, Editor(s)

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