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Proceedings Paper

Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging
Author(s): David Moreau; Claire Lefort; Sylvia M. Bardet; Rodney P. O'Connor
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Paper Abstract

Infrared laser light radiation can be used to depolarize neurons and to stimulate neural activity. The absorption of infrared radiation and heating of biological tissue is thought to be the underlying mechanism of this phenomenon whereby local temperature increases in the plasma membrane of cells either directly influence membrane properties or act via temperature sensitive ion channels. Action potentials are typically measured electrically in neurons with microelectrodes, but they can also be observed using fluorescence microscopy techniques that use synthetic or genetically encoded calcium indicators. In this work, we studied the impact of infrared laser light on neuronal calcium signals to address the mechanism of these thermal effects. Cultured primary mouse hippocampal neurons expressing the genetically encoded calcium indicator GCaMP6s were used in combination with the temperature sensitive fluorophore Rhodamine B to measure calcium signals and temperature changes at the cellular level. Here we present our all-optical strategy for studying the influence of infrared laser light on neuronal activity.

Paper Details

Date Published: 9 March 2016
PDF: 8 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96901M (9 March 2016); doi: 10.1117/12.2211355
Show Author Affiliations
David Moreau, Univ. of Limoges, XLIM Institut de Recherche, CNRS (France)
Claire Lefort, Univ. of Limoges, XLIM Institut de Recherche, CNRS (France)
Sylvia M. Bardet, Univ. of Limoges, XLIM Institut de Recherche, CNRS (France)
Rodney P. O'Connor, Univ. of Limoges, XLIM Institut de Recherche, CNRS (France)


Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

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