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Proceedings Paper

Fabrication of Indocyanine Green and 2H, 3H-perfluoropentane loaded microbubbles for fluorescence and ultrasound imaging
Author(s): Yutong He; Qiang Wu; Rong Ma; Shufang Chang; Pengfei Shao; Ronald Xu
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Paper Abstract

As a near-infrared (NIR) fluorescence dye, Indocyanine Green (ICG) has not gained broader clinical applications, owing to its multiple limitations such as concentration-dependent aggregation, low fluorescence quantum yield, poor physicochemical stability and rapid elimination from the body. In the meanwhile, 2H,3H-perfluoropentane (H-PFP) has been widely studied in ultrasound imaging as a vehicle for targeted delivery of contrast agents and drugs. We synthesized a novel dual-modal fluorescence and ultrasound contrast agent by encapsulating ICG and H-PFP in lipid microbubbles using a liquid-driven coaxial flow focusing (LDCFF) process. Uniform microbubbles with the sizes ranging from 1-10um and great ICG loading efficiency was achieved by this method. Our benchtop experiments showed that ICG/H-PFP microbubbles exhibited less aggregation, increased fluorescence intensity and more stable photostability compared to free ICG aqueous solution. Our phantom experiments demonstrated that ICG/H-PFP microbubbles enhanced the imaging contrasts in fluorescence imaging and ultrasonography. Our animal experiments indicated that ICG/H-PFP microbubbles extended the ICG life time and facilitated dual mode fluorescence and ultrasound imaging in vivo.

Paper Details

Date Published: 10 March 2016
PDF: 9 pages
Proc. SPIE 9701, Multimodal Biomedical Imaging XI, 97010T (10 March 2016); doi: 10.1117/12.2211333
Show Author Affiliations
Yutong He, Univ. of Science and Technology of China (China)
Qiang Wu, Univ. of Science and Technology of China (China)
Rong Ma, Chongqing Medical Univ. (China)
Shufang Chang, Chongqing Medical Univ. (China)
Pengfei Shao, Univ. of Science and Technology of China (China)
Ronald Xu, Univ. of Science and Technology of China (China)
Chongqing Medical Univ. (China)
The Ohio State Univ. (United States)


Published in SPIE Proceedings Vol. 9701:
Multimodal Biomedical Imaging XI
Fred S. Azar; Xavier Intes, Editor(s)

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