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Proceedings Paper

Tracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope
Author(s): L. Streppa; L. Berguiga; E. Boyer Provera; F. Ratti; E. Goillot; C. Martinez Torres; L. Schaeffer; Juan Elezgaray; A. Arneodo; F. Argoul
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Paper Abstract

We introduce a high resolution scanning surface plasmon microscope for long term imaging of living adherent mouse myoblast cells. The coupling of a high numerical aperture objective lens with a fibered heterodyne interferometer provides both enhanced sensitivity and long term stability. This microscope takes advantage of the plasmon resonance excitation and the amplification of the electromagnetic field in near-field distance to the gold coated coverslip. This plasmon enhanced evanescent wave microscopy is particularly attractive for the study of cell adhesion and motility since it can be operated without staining of the biological sample. We show that this microscope allows very long-term imaging of living samples, and that it can capture and follow the temporal deformation of C2C12 myoblast cell protusions (lamellipodia), during their migration on a at surface.

Paper Details

Date Published: 22 April 2016
PDF: 10 pages
Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 97240G (22 April 2016); doi: 10.1117/12.2211331
Show Author Affiliations
L. Streppa, Ecole Normale Supérieure de Lyon (France)
Univ. de Lyon (France)
L. Berguiga, Univ. de Lyon (France)
Ecole Normale Supérieure de Lyon (France)
E. Boyer Provera, Ecole Normale Supérieure de Lyon (France)
Univ. de Lyon (France)
F. Ratti, Univ. de Lyon (France)
Ecole Normale Supérieure de Lyon (France)
E. Goillot, Univ. de Lyon (France)
Ecole Normale Supérieure de Lyon (France)
C. Martinez Torres, Ecole Normale Supérieure de Lyon (France)
Univ. de Lyon (France)
L. Schaeffer, Univ. de Lyon (France)
Ecole Normale Supérieure de Lyon (France)
Juan Elezgaray, Institut de Chimie et Biologie des Membranes et des Nano-objets (France)
Univ. de Bordeaux (France)
A. Arneodo, Ecole Normale Supérieure de Lyon (France)
Univ. de Lyon (France)
Univ. de Bordeaux (France)
F. Argoul, Ecole Normale Supérieure de Lyon (France)
Univ. de Lyon (France)
Univ. de Bordeaux (France)


Published in SPIE Proceedings Vol. 9724:
Plasmonics in Biology and Medicine XIII
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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