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Proceedings Paper

Study on fluorescence spectra of thiamine, riboflavin and pyridoxine
Author(s): Hui Yang; Xue Xiao; Xuesong Zhao; Lan Hu; Caofang Lv; Zhangkun Yin
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Paper Abstract

This paper presents the intrinsic fluorescence characteristics of vitamin B1, B2 and B6 measured with 3D fluorescence Spectrophotometer. Three strong fluorescence areas of vitamin B2 locate at λex/λem=270/525nm, 370/525nm and 450/525nm, one fluorescence areas of vitamin B1 locates at λex/λem=370/460nm, two fluorescence areas of vitamin B6 locate at λex/λem=250/370nm and 325/370nm were found. The influence of pH of solution to the fluorescence profile was also discussed. Using the PARAFAC algorithm, 10 vitamin B1, B2 and B6 mixed solutions were successfully decomposed, and the emission profiles, excitation profiles, central wavelengths and the concentration of the three components were retrieved precisely through about 5 iteration times.

Paper Details

Date Published: 26 January 2016
PDF: 10 pages
Proc. SPIE 9903, Seventh International Symposium on Precision Mechanical Measurements, 99030H (26 January 2016); doi: 10.1117/12.2211248
Show Author Affiliations
Hui Yang, New Star Application Technology Institute (China)
Xue Xiao, Anhui Institute of Optics and Fine Mechanics (China)
Xuesong Zhao, Anhui Institute of Optics and Fine Mechanics (China)
Lan Hu, New Star Application Technology Institute (China)
Caofang Lv, New Star Application Technology Institute (China)
Zhangkun Yin, New Star Application Technology Institute (China)


Published in SPIE Proceedings Vol. 9903:
Seventh International Symposium on Precision Mechanical Measurements
Liandong Yu, Editor(s)

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