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Proceedings Paper

A light sheet confocal microscope for image cytometry with a variable linear slit detector
Author(s): Joshua A. Hutcheson; Foysal Z. Khan; Amy J. Powless; Devin Benson; Courtney Hunter; Ingrid Fritsch; Timothy J. Muldoon
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Paper Abstract

We present a light sheet confocal microscope (LSCM) capable of high-resolution imaging of cell suspensions in a microfluidic environment. In lieu of conventional pressure-driven flow or mechanical translation of the samples, we have employed a novel method of fluid transport, redox-magnetohydrodynamics (redox-MHD). This method achieves fluid motion by inducing a small current into the suspension in the presence of a magnetic field via electrodes patterned onto a silicon chip. This on-chip transportation requires no moving parts, and is coupled to the remainder of the imaging system. The microscopy system comprises a 450 nm diode 20 mW laser coupled to a single mode fiber and a cylindrical lens that converges the light sheet into the back aperture of a 10x, 0.3 NA objective lens in an epi-illumination configuration. The emission pathway contains a 150 mm tube lens that focuses the light onto the linear sensor at the conjugate image plane. The linear sensor (ELiiXA+ 8k/4k) has three lateral binning modes which enables variable detection aperture widths between 5, 10, or 20 μm, which can be used to vary axial resolution. We have demonstrated redox-MHD-enabled light sheet microscopy in suspension of fluorescent polystyrene beads. This approach has potential as a high-throughput image cytometer with myriad cellular diagnostic applications.

Paper Details

Date Published: 7 March 2016
PDF: 6 pages
Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200U (7 March 2016); doi: 10.1117/12.2211164
Show Author Affiliations
Joshua A. Hutcheson, Univ. of Arkansas (United States)
Foysal Z. Khan, Univ. of Arkansas (United States)
Amy J. Powless, Univ. of Arkansas (United States)
Devin Benson, Univ. of Arkansas (United States)
Courtney Hunter, Univ. of Arkansas (United States)
Ingrid Fritsch, Univ. of Arkansas (United States)
Timothy J. Muldoon, Univ. of Arkansas (United States)


Published in SPIE Proceedings Vol. 9720:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management
Kevin K. Tsia; Keisuke Goda, Editor(s)

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