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Proceedings Paper

Three-dimensional imaging of intracochlear tissue by scanning laser optical tomography (SLOT)
Author(s): N. Tinne; L. Nolte; G. C. Antonopoulos; J. Schulze; J. Andrade; A. Heisterkamp; H. Meyer; A. Warnecke; O. Majdani; T. Ripken
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Paper Abstract

The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure.

Paper Details

Date Published: 29 February 2016
PDF: 6 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96892H (29 February 2016); doi: 10.1117/12.2211066
Show Author Affiliations
N. Tinne, Laser Zentrum Hannover e.V. (Germany)
Cluster of Excellence "Hearing4all" (Germany)
L. Nolte, Laser Zentrum Hannover e.V. (Germany)
G. C. Antonopoulos, Laser Zentrum Hannover e.V. (Germany)
J. Schulze, Medizinische Hochschule Hannover (Germany)
Cluster of Excellence "Hearing4all" (Germany)
J. Andrade, Medizinische Hochschule Hannover (Germany)
A. Heisterkamp, Laser Zentrum Hannover e.V. (Germany)
Leibniz Univ. Hannover (Germany)
Cluster of Excellence "Hearing4all" (Germany)
H. Meyer, Laser Zentrum Hannover e.V. (Germany)
A. Warnecke, Medizinische Hochschule Hannover (Germany)
Cluster of Excellence "Hearing4all" (Germany)
O. Majdani, Medizinische Hochschule Hannover (Germany)
Cluster of Excellence "Hearing4all" (Germany)
T. Ripken, Laser Zentrum Hannover e.V. (Germany)
Cluster of Excellence "Hearing4all" (Germany)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong; Justus F. Ilgner; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory; Laura Marcu; Haishan Zeng, Editor(s)

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