Share Email Print

Proceedings Paper

Optical elastic scattering for early label-free identification of clinical pathogens
Author(s): Valentin Genuer; Olivier Gal; Jérémy Méteau; Pierre Marcoux; Emmanuelle Schultz; Éric Lacot; Max Maurin; Jean-Marc Dinten
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We report here on the ability of elastic light scattering in discriminating Gram+, Gram- and yeasts at an early stage of growth (6h). Our technique is non-invasive, low cost and does require neither skilled operators nor reagents. Therefore it is compatible with automation. It is based on the analysis of the scattering pattern (scatterogram) generated by a bacterial microcolony growing on agar, when placed in the path of a laser beam. Measurements are directly performed on closed Petri dishes.

The characteristic features of a given scatterogram are first computed by projecting the pattern onto the Zernike orthogonal basis. Then the obtained data are compared to a database so that machine learning can yield identification result. A 10-fold cross-validation was performed on a database over 8 species (15 strains, 1906 scatterograms), at 6h of incubation. It yielded a 94% correct classification rate between Gram+, Gram- and yeasts. Results can be improved by using a more relevant function basis for projections, such as Fourier-Bessel functions. A fully integrated instrument has been installed at the Grenoble hospital’s laboratory of bacteriology and a validation campaign has been started for the early screening of MSSA and MRSA (Staphylococcus aureus, methicillin-resistant S. aureus) carriers.

Up to now, all the published studies about elastic scattering were performed in a forward mode, which is restricted to transparent media. However, in clinical diagnostics, most of media are opaque, such as blood-supplemented agar. That is why we propose a novel scheme capable of collecting back-scattered light which provides comparable results.

Paper Details

Date Published: 7 March 2016
PDF: 13 pages
Proc. SPIE 9698, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV, 96980A (7 March 2016); doi: 10.1117/12.2211021
Show Author Affiliations
Valentin Genuer, Univ. Grenoble-alpes (France)
CEA, LETI (France)
Olivier Gal, CEA, LIST (France)
Jérémy Méteau, Univ. Grenoble-alpes (France)
CEA, LETI (France)
Pierre Marcoux, Univ. Grenoble-alpes (France)
CEA, LETI (France)
Emmanuelle Schultz, Univ. Grenoble-alpes (France)
CEA, LETI (France)
Éric Lacot, Univ. Grenoble-alpes (France)
Liphy (France)
Max Maurin, CHU de Grenoble (France)
Jean-Marc Dinten, Univ. Grenoble-alpes (France)
CEA, LETI (France)

Published in SPIE Proceedings Vol. 9698:
Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

© SPIE. Terms of Use
Back to Top