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Proceedings Paper

Experimental assessment of thermal effects of high power density light stimulation for optogenetics control of deep brain structures (Conference Presentation)
Author(s): Suhan Senova; Ilona Scisniak; Chih Chieh Chiang; Isabelle Doignon; Claire Martin; Stephane Palfi; Antoine Chaillet; Frederic Pain

Paper Abstract

2D surface maps of light distribution and temperature increase were recorded in wild type anesthetized rats brains during 90s light stimulation at 478nm (blue) and 638nm (red) with continuous or pulsed optical stimulations with corresponding power ranging from 100 up to 1200 mW/mm² at the output of an optical fiber. Post mortem maps were recorded in the same animals to assess the cooling effect of blood flow. Post mortem histological analysis were carried out to assess whether high power light stimulations had phototoxic effects or could trigger non physiological functional activation. Temperature increase remains below physiological changes (0,5 -1°) for stimulations up to 400mW/mm² at 40Hz. . Histology did not show significant irreversible modifications or damage to the tissues. The spatial profile of light distribution and heat were correlated and demonstrate as expected a rapid attenuation with diatnce to the fiber.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96902H (26 April 2016); doi: 10.1117/12.2210944
Show Author Affiliations
Suhan Senova, C.H.U. Henri-Mondor (France)
INSERM (France)
Univ. Paris-EST (France)
Ilona Scisniak, Univ. Paris Sud (France)
Univ. Paris Saclay (France)
Chih Chieh Chiang, National Tsing Hua Univ. (Taiwan)
Isabelle Doignon, Université Paris Sud INSERM, Laboratoire Interactions Cellulaires et PhysiologieHépatique (France)
Claire Martin, Institut National de Physique Nucléaire et de Physique des Particules (France)
Univ. Paris Saclay (France)
Ctr. National de la Recherche Scientifique (France)
Stephane Palfi, C.H.U. Henri-Mondor (France)
INSERM (France)
Univ. Paris-Est Crétail Val de Marne (France)
Antoine Chaillet, Univ. Paris-Sud (France)
Univ. Paris Saclay (France)
Supélec (France)
Frederic Pain, Univ. Paris Sud (France)
Univ. Paris Saclay (France)
Ctr. National de la Recherche Scientifique (France)


Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

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